Home
Creators
Search Recent Missing Random
Posts
Search DMs Popular Hash Lookup Tags Random
Importer
Import FAQ
Register Favorites Keys Review DMs Logout
Community
Telegram ThePornDude
Home Creators Posts Import Register Favorites Logout

Using Cryogel to keep mammalian cells alive at RT (Patreon)

Published:
2018-02-19 16:17:15
Imported:
2026-06
haven't archived this post yet. have a subscription? use the importer!

Content

 

Storage and transport is a vital part of any lab that does cell culture, but for anything other than bacteria and fungi, the ways to do this require extreme cold. Here we demonstrate how to prepare a ultralight foam from cross linked gelatin, which can be used to keep cells alive for up to a week at room temperature. No incubator, CO2, dry ice or liquid nitrogen required. The material is easy to make and prepare and the method works for both adherent and suspension cells.

This was filmed while my friend was letting me do the lactose project in his lab. Since filming, he's used this method to ship cells and they survived and were able to be cultured.


Files

Previews only

Using DIY CRYOGEL to Keep Mammalian Cells Alive at Room Temp

Storage and transport is a vital part of any lab that does cell culture, but for anything other than bacteria and fungi, the ways to do this require extreme cold. Here we demonstrate how to prepare a ultralight foam from cross linked gelatin, which can be used to keep cells alive for up to a week at room temperature. No incubator, CO2, dry ice or liquid nitrogen required. The material is easy to make and prepare and the method works for both adherent and suspension cells. This was filmed while my friend was letting me do the lactose project in his lab. Since filming, he's used this method to ship cells and they survived and were able to be cultured. Link to original paper: https://www.nature.com/articles/srep41551 __________________________________________________________________ Interested in some sweet science themed designs for your wardrobe or wall? Click the link below to see all the great items currently being offered: https://www.redbubble.com/people/chironex?asc=u __________________________________________________________________ Want to support the show and my research? Check out my patreon at: https://www.patreon.com/thethoughtemporium __________________________________________________________________ My Social Media Pages: Instagram: https://www.instagram.com/thethoughtemporium/ Facebook: https://www.facebook.com/thethoughtemporium/ Twitter: https://www.twitter.com/TTEchironex Website: http://thethoughtemporium.com/ __________________________________________________________________ As always, thanks to my awesome Patrons for helping to make these video possible. Thanks to: -John De Witt -Paul Emmerich -Terry Fuller -Jason Dunham -Anita Fowler -Jonas Abreu -Alex Lemna -Orotusso -cataract bumblesnatch

Comments

Jasper

So how does it work? The article seems to say it mechanically, and different structures fit different (shapes of)cells. Also mentions adherence of the cells and that it is "soft". Admittedly haven't read the entire paper. Would (fairly naively)have guessed the above, or reducing diffusion away from the cell might help.

thethoughtemporium
>>10254732

There's a couple mechanisms at play. The protein structure of the cryogel has lots of functional groups for the cells to adhere to, so they don't freak out because they think they're floating (normally that makes cells explode if left like that). The little pockets also sort of envelop the cells which helps to protect them. The adhesion isn't that strong though so they let go when you transfer the mix to a fresh flask. Basically it tricks the cells into thinking they're in some big chunk of tissue, so they don't panic.