Extracting RFP (Patreon)

Extracting Red Fluorescent Protein Using Ultrasound

The natural world is full of colorful molecules, but few have been used by biologist more than green fluorescent protein and it's beta barrel relatives. Fluorescent proteins come in a literal rainbow of colors and can be both natural and/or artificial in origin. Last week we built an awesome little bioreacotr and with it we grew 400ml of E coli that produce a cousin of GFP, Red Fluorescent Protein. In this video we go through the basics of protein extraction and show how you go from growth media to final product. I'll be sending a sample of the purified extract to Nurdrage so he can test and see if fluorescent proteins are viable dyes for glowstick chemistry. We're not expecting it to be very good, but even a flash of light for a moment would be very interesting. Lantern: https://www.youtube.com/watch?v=l_b0SCabjvo GFP Mead: https://www.youtube.com/watch?v=LOyBYEks550 Ultrasound: https://www.youtube.com/watch?v=iRh2oeB2OhQ Sebastian's Page: https://www.instagram.com/atinygreencell _________________________________________________________________ Support the show and future projects: Patreon: https://www.patreon.com/thethoughtemporium Store: https://teespring.com/stores/the-thought-emporium Become a member: https://www.youtube.com/channel/UCV5vCi3jPJdURZwAOO_FNfQ/join _________________________________________________________________ My Social Media Pages: Instagram: https://www.instagram.com/thethoughtemporium/ Facebook: https://www.facebook.com/thethoughtemporium/ Twitter: https://www.twitter.com/TTEchironex Website: http://thethoughtemporium.com/ _________________________________________________________________ As always, thanks to my awesome Patrons for helping to make these video possible. Special thanks to: Afrotechmods Alex Alex Vanderpot Alexander Halbleib Alexandre Guhur Andrew Gray Anita Fowler Austin Morris Ben Krasnow Ben Reay Bennet Huch besenyeim Cataract Bumblesnatch Christian Bastos Colby Callahan Danny Chan Daniel Gunn David Golouski Ezekiel Dohmen Elliot Turner Frank Harlad Havana Woody Jase Smith John De Witt Jim Mussared Jonas Abreu Jonathan Anderson Justin Hendryx Kevin Kevin Comerford Kevin Forsythe kn0tsin Kush Agarwal Larry Leon Schutte Matt KI7ZTH Michael Chatzidakis narvutar1324 Nico Schlüter Nicolas Pourtalé Noah Pedersen OmuN Patrick Sweetman Patryk Wycech Paul Emmerich Paul Richmond Peter Scott Philipp Weber Pietro Saccardi Rauni Robert S. Ryan Harvey Simon Hallam Skave Rat Tom Bullock tain TheQuickestBrownFox Vasilis Whence __________ Channel Members: Felix Nica L Weiner Kush Agarwal Anthony M Hunter Bagby