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 Genetic modification sounds like something that would be very complicated and difficult to do but the reality is that couldn't be further from the truth. With the right tools and techniques it's something that can be done on your kitchen table if you really wanted. In this video we explore how bacteria can easily be modified to produce a range of fluorescent proteins and even bioluminesce.  

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Genetically Modify Bacteria In 3 Simple Steps - Heat shock

Genetic modification sounds like something that would be very complicated and difficult to do but the reality is that couldn't be further from the truth. With the right tools and techniques it's something that can be done on your kitchen table if you really wanted. In this video we explore how bacteria can easily be modified to produce a range of fluorescent proteins and even bioluminesce. More resources: Building an incubator/heatblock - https://youtu.be/Zm378x6zJYc DNA Extraction - https://youtu.be/knosqmvLWSc Running a Gel - https://youtu.be/sSKls2kNC4U Building a Gel Doc - https://youtu.be/dSqt073HyE0 DNA transcription and translation - https://www.youtube.com/watch?v=itsb2SqR-R0 Spider silk project overview - https://youtu.be/Fx8TcGrCOSI Sebastians pages: Instagram - https://www.instagram.com/atinygreencell/ Bionomica labs - https://binomicalabs.org/ David: Channel - https://www.youtube.com/channel/UCftNyK6c9JdIFTQ2TLQqm_g Stores to buy reagents: http://bio-world.com/ https://www.phytotechlab.com/ https://www.goldbio.com/ https://www.carolina.com/ http://amino.bio/ https://www.addgene.org/ http://neb.com/ _________________________________________________________________ Support the show and future projects: Patreon: https://www.patreon.com/thethoughtemporium Ko-Fi: https://ko-fi.com/thoughtemporium Become a member: https://www.youtube.com/channel/UCV5vCi3jPJdURZwAOO_FNfQ/join Store: https://teespring.com/stores/the-thought-emporium _________________________________________________________________ My Social Media Pages: Instagram: https://www.instagram.com/thethoughtemporium/ Facebook: https://www.facebook.com/thethoughtemporium/ Twitter: https://www.twitter.com/TTEchironex Website: http://thethoughtemporium.com/ _________________________________________________________________ Special thanks to my amazing patrons and channel members! Afrotechmods Alexander Eisele Alexander Marunowski Alexandre Guhur Ambrose Andre Andrei-Gabriel Andrei-Gabriel andrew james morris Andy Anita Fowler Applied Science Austin Morris Ben Ben Reay Bennet Huch besenyeim BinarySplit bluejay Brad Walker Brady OBrien Chris the Mad Sciencer Comrade Spamuel Cronenberg Jerry Danny Chan Dave Yeagly David Choitz Dean Pierce Dima Drew DeVault dstensnes Dustin Parciasepe Elliot Turner emptymachine ethan lutz Eugene Pakhomov Ezekiel Dohmen Filipsi Frank Gal Tibi Harry Pottash ian burghardt illia Jack Brown James Jaroslav Henner Jase Smith Jim Mussared John John De Witt Jon Adams Jonas Abreu Jonathan Dashe Joshua Pedrick Justa Noman Justin Hendryx Kevin Kevin Forsythe kn0tsin Krys Kamieniecki Lambda GPU Workstations Lee Goymer Leon Leon Schutte Leslie Rohde Liam Scaife Louis Cashin Luc Ritchie Marco Reps Martin Haugsand Matthew Broerman Matthew Reece-Ford Maxwell Meinhard Absalon Michael Chatzidakis Michael Duran Michael Smith Mike Miller Monsyne Dragon narvutar1324 Nicholas Fletcher Nico Schlüter Owen Ashurst Owen Burns Patreon Patrick Sweetman Paul Emmerich Paul Richmond Paul Toensing Philipp Weber Pietro Saccardi Piper Elinor Rae McIntosh Rajan Chari Ralph Dratman Rauni Rikki Guy Robert Boll robert braun Robert Miller Robert S. Ruben Sam Pinches sdrwer erwer Sean Coates Sebastiaan Schlappi Simon Convey Simon Hallam SkaveRat Slop Shop Stephen Anderson tain TheOneTrueJames TheQuickestBrownFox Tiffany Bennett Toli Tom Bullock Tómas Árni Jónasson Valery Belayev Vedran Bajic Virgil Ilian Walter Jones xj9 Yannik Neuhaus Zwaluw Áva Eriksdóttir Channel members: Jeko Ddragon BasiC7786 Roman Cerny Jacob Stewart steven hammel Mellonoma The Great Paweł Michalak bitizen248 Moritz Imen Ainius Beriozovas Emi Li Incognito AI abhijith raghu Faris Faux Matt Ettler Chris Modjeska andré bernard mennicken Jose Ayala Shwe Yin Aye Radio Astronomy (MSAS) stevenson primacio Ajit A Guillermo Alum flantc ProjectNightingaleForResponsibleMedicine Dr. Brandon Wiley Jonathan Nagel István Kiss Jorm Skagh txyzinfo Robert Boll trevor skjerpen adamklam1 Herbling Anthony M Kush Agarwal

Comments

Leslie Rohde

Justin, great stuff as always, and glad to see you back on the bio-track. Based on the brief views of your frig and freezer, maybe you have the problem I do and (I hope) have a suggestion or two about how to deal with it. - Bio vials are very small, so labeling them is a challenge. - It's nearly impossible not to get some alcohol on them when creating a sterile field, which takes off (so called) permanent marker. - Add to that the verity of sizes and my storage is a MESS! You got any secrets on organizing??

thethoughtemporium

Printed labels can help. Like old fashioned label maker. Otherwise you need to be careful to constantly relabel thing. Other than that, switch from alcohol to something like chlorhexdin or maybe bleach for setting up a sterile field. Beyond that I like tupperware containers to sort all my stuff. Self contained boxes based on use. Enzymes, stabs, etc. That and taking a day from time to time to resort everything and make room as needed.

Luc Ritchie

I swear every single one of your videos is cooler than the rest of what's on YouTube combined. I know you've spoken in the past on why DIYing equipment isn't a great idea a lot of the time, and is more of a time sink (and quality hole) than it's worth. But from the perspective of someone who enjoys the DIY for its own sake, is there anything in particular about centrifuges that makes them particularly unsuited to DIY (e.g. quality control)?

thethoughtemporium

DIY fuges are a death trap. They're spinning so fast that unless they're exceptionally well built, if something fails while they're running they could throw a tube, or the entire rotor at you while spinning at up to 12000rpm. Even proper ones are handled with extreme care for that reason and why we always take great care to balance them before ever turning them on. I'm not saying it's impossible, but for the price of materials to make one that's good and safe, it's better to buy one